When this method is suitable, it is inefficient to use pairwise comparisons because pairwise confidence intervals are wider and the hypothesis tests are less powerful for a given confidence level. Sample size calculation in multiple comparison procedures. A bonferroni multiple comparisons procedure using a statistical software package yields the significance levels pvalues, adjusted to be interpreted according to the usual 5%. Multiple comparison procedures pdf free download epdf. A comparative study on multiple comparison procedures. Also see sections of this book with the terms multiple comparisons, tukey, pairwise, posthoc, p. The hochberg and hommel methods provide power gains for the analysis, albeit small, in comparison to the bonferroni method. The most versatile of the post hoc multiplecomparison tests is the scheffe procedure, which allows any number of post hoc contrasts. The concept of consistency is introduced and discussed in relation to five of the more common multiple comparison procedures. In general, when an analytical null model is available, you should use it to compute pvalues and then use the benjamini hochberg procedure. Fdrcontrolling procedures are designed to control the expected proportion of discoveries rejected null hypotheses that are false incorrect rejections. Westfall, mail stop 2101, texas tech university, lubbock tx 79409 randall d. Multiple comparison procedures multiple comparison procedures yosef hochberg telaviv university ramataviv, israel.
Multiple comparisons handbook of biological statistics. The resulting mc test rejects the null hypothesis if, and only if, at least one pair of mc intervals does not overlap. The multtest procedure will perform a number of multiple testing procedures. Although multiple comparisons are often conducted in association with anova, they are in fact standalone procedures hsu 1996. Using multiple comparisons to assess differences in group. Remarks comparison to other multiple test procedures. It works with raw data for anova models, and can also accept a list of pvalues as shown here. If we form two 95%confidence intervals for two means or two effect differences, etc. R has built in methods to adjust a series of pvalues either to. Multiple comparisons tests mcts are performed several times on the mean of experimental conditions. A simple sequentially rejective multiple test procedure. Tamhane, phd, is senior associate dean of the mccormick school of engineering and applied science at northwestern university, where he is also professor in the department of industrial engineering and management sciences. The holm 1979 and hochberg 1988 methods begin by computing the values where jp j, which are 0. The control of the false discovery rate in multiple testing under dependency.
Examining multiple comparison procedures according to error rate. The term multiple comparison refers to the individual comparison of two means selected from a larger set of means. Keywords bonferroni type procedures, metaanalysis, multicenter, multiple comparison procedures, pvalues, stepwise procedures, subset analysis references benjamini, y, hochberg, y the adaptive control of the false discovery rate in multiple independent testing problems series in statistics 93. Moreover we posit that the problem of multiple comparisons can disappear entirely when viewed from a hierarchical bayesian perspective. Choose with a control to compare the level means to the mean of a control group. The false discovery rate fdr is a method of conceptualizing the rate of type i errors in null hypothesis testing when conducting multiple comparisons. A comparison of procedures for multiple comparisons of means with unequal variances authors. Methods to adjust for multiple comparisons in the analysis. In largescale multiple testing as often happens in genomics, you may be better served by controlling the false discovery rate fdr. If you increase the number of tests, and the distribution of p values is the same in the newly added tests as in the original tests, the benjaminihochberg procedure will yield the same proportion of significant results. Controlling error in multiple comparisons, with examples. For pairwise comparisons after the data have been analyzed, duncans multiplerange test seems to be at least as powerful as any other, and is perhaps the most frequently used such test. Classical procedures for the test problems above like of tukcy, schcffc, 505.
The stepdownminp procedure performs well for complete data. Comparisons of methods for multiple hypothesis testing in. In 1996, the first conference on multiple comparisons took place in israel. Implicit in all multiple testing procedures is the assumption that the. Basic concepts and notation this paper assumes that the reader is familiar with the key concepts in the theory of multiple comparisons. For more information about multiple comparison procedures, see hochberg and. On the adaptive control of the false discovery rate in. Hochberg procedure controls the fdr at level less than or equal to m 0. We shall refer to this procedure as the s procedure. Tamhane offering a balanced, uptodate view of multiple comparison procedures, this book refutes the belief held by some statisticians that such procedures have no place in data analysis. A comparison of procedures for multiple comparisons of.
Pdf the control of the false discovery rate in multiple. Multiple comparisons when comparing more than two means, an anova ftest tells you whether the means are significantly different from each other, but it does not tell you which means differ from which other means. This module computes sample sizes for multiple comparison procedures. The method starts with a set of pvalues which are assumed to be independent and we select a threshold for the fdr, which we denote q. Multiple comparison procedures mcps, also called mean separation tests, give you more detailed information about the differences among the means. We propose building multilevel models in the settings where multiple comparisons arise. What is the proper way to apply the multiple comparison test. A modified benjamini hochberg multiple comparisons procedure for controlling the false discovery rate article in journal of statistical planning and inference 1042. Some general theory of multiple comparison procedures pages 343361 summary. The benjaminihochberg procedure is less sensitive than the bonferroni procedure to your decision about what is a family of tests. Now that we have introduced both p values and hypothesis tests, we need to spend a little time grappling with the problem of multiple comparisons.
Methods for addressing multiple testing fwer and fdr. Multiple comparison procedures wiley series in probability and statistics yosef hochberg, ajit c. The goal in multiple comparisons is to compare the average effects of three or more treatments for example, drugs, groups of subjects to decide which treatments are better, which ones are worse. We show that both are special cases of partition testing. Sas stat users guide, 1990 the closed test procedure is recommcnded to be more powerful than many other multiple test procedures including the multiple stage methods. The topic of multiple comparison procedures mcps is receiving nowadays a growing theoretical. In statistics, the multiple comparisons, multiplicity or multiple testing problem occurs when one. Hochberg 1976 proposed the following procedure based on a generalization of the tukey method of multiple comparisons. More powerful procedures for multiple significance testing. Nonparametric pairwise multiple comparisons in independent groups using dunns test alexis dinno school of community health portland state university portland, or alexis. Yosef hochberg, phd, is professor in the department of statistics and operations research at tel aviv university, israel. Using false discovery rates for multiple comparisons in. However, it removes participants with missing values prior to the analysis resulting in a loss of power when there are missing data. Dunns test is the appropriate nonparametric pairwise multiple comparison procedure following rejection of a kruskalwallistest, and is now imple.
The bonferroni and holm methods provide the same disjunctive power. Hochberg s areas of research interest include multiple comparisons, categorical data analysis, and medical applications of biostatistical methods. Multilevel models perform partial pooling shifting estimates toward each other, whereas classical procedures typically keep. Multiple comparison procedures wiley series in probability and. Multiple comparison procedures yosef hochberg, ajit c. This regular article is brought to you for free and open access by the open access. Three alternative procedures to adjust significance levels for multiplicity are the traditional bonferroni technique, a sequential bonferroni technique developed by hochberg 1988, and a sequential approach for controlling the false discovery rate proposed by benjamini and hochberg 1995. Available at a lower price from other sellers that may not offer free prime. Hochbergs areas of research interest include multiple comparisons, categorical data analysis, and medical applications of biostatistical methods. Implicit in all multiple testing procedures is the assumption that the distribution of pvalues is correct this assumption often is not valid for genomics data where pvalues are obtained by asymptotic theory thus, resampling methods are often used to calculate calculate pvalues.
Nonparametric pairwise multiple comparisons in independent. View table of contents for multiple comparison procedures. Other methods, such as the closed testing procedure marcus et al. Why we usually dont have to worry about multiple comparisons andrew gelmany, jennifer hillz, masanao yajimax april 21, 2008 abstract the problem of multiple comparisons can disappear when viewed from a bayesian perspective. Benjamini hochberg control of fdr the benjamini hochberg adjustment is a step down procedure, i. These are potential adjusted p values, determined ultimately by the stepwise procedures. Multiple comparison procedures available in multcomp. The fdr works by estimating some rejection region so that, on average, fdr multiple comparisons procedures fdr simesequality hochberg s procedure mtp2 densities positive regression dependency unidimensional latent variables discrete test statistics multiple endpoints manytoone comparisons comparisons with control. A sharper bonferroni procedure for multiple tests of significance. Saville a practicing statistician looks at the multiple comparison controversy and related issues through the eyes of the users. Note that fdr false discovery rate is the name that benjamini and hochberg give to their procedure and that this nomenclature is used by sas.
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